MDH1 anticorps
Aperçu rapide pour MDH1 anticorps (ABIN568310)
Antigène
Voir toutes MDH1 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
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Purification
- Ammonium Sulphate Precipitation and Ion Exchange Chromatography
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Immunogène
- Malic dehydrogenase is isolated and purified from Human erythrocytes. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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Isotype
- IgG
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Reconstitution
- Restore by adding 1.0 mL of sterile distilled water
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Concentration
- 10.0 mg/mL
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Buffer
- PBS, pH 7.2 without preservatives and foreign proteins
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Agent conservateur
- Without preservative
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
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- MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))
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Autre désignation
- MDHA
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Sujet
- Malate dehydrogenase catalyzes the interconversion of L-malate and oxaloacetate using nicotinamide adenine dinucleotide (NAD) as a coenzyme. Malate dehydrogenase is found in all eukaryotic cells as two isozymes: mitochondrial (m-MDH) and cytoplasmic (soluble, s-MDH). Prokaryotes contain only a single form. The two isozymes, both consisting of two very similar subunits of about 35kD and having similar enzymatic activity appear as different proteins. There is structural similarity of mitochondrial malate dehydrogenase to L-3-hydroxyacyl CoA dehydrogenase and the cytoplasmic malate dehydrogenase to lactate dehydrogenase.Synonyms: MDH1, Malate dehydrogenase, cytoplasmic
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ID gène
- 4190
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NCBI Accession
- NP_005908
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UniProt
- P40925
Antigène
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